Two chemometric methods were developed for the simultaneous determination of metoprolol succinate and hydrochlorothiazide. The chemometric methods applied were principal component regression (PCR) and partial least square (PLS). These approaches were successfully applied to quantify the two drugs in the mixture using the information in the UV absorption spectra of appropriate solutions in the range of 220-275 nm with the interval ∆λ = 1 nm. The model development (calibration) was carried out with set of 25 mixtures and model validation with set of 10 mixtures. The evaluation of calibration model is relied on the coefficient of determination (R2) and root mean square error of calibration (RMSEC). The coefficient of determination (R2) for the relationship between actual values and predicted values of both drugs was higher than 0.995 indicating good accuracy of the developed method while RMSEC values obtained were relatively low which indicate acceptable precision of analytical method. The PCR and PLS methods require neither any separation step, nor any prior graphical treatment of the overlapping spectra of the two drugs in a mixture. The proposed methods are simple, rapid and can be easily used as an alternative analysis tool in the quality control as well as in process control of drugs and formulation.
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